UV detection measures the total flavonolignans content as silymarin, which is a traditional, cost-effective method widely used for routine quality control. It reflects the overall active content but cannot distinguish individual components.
HPLC testing can separate and quantify the main active isomers, such as silybin A, silybin B, isosilybin, silychristin, and silydianin. It offers higher accuracy, specificity, and repeatability, and is usually required for high-end products, pharmaceutical preparations, and USP/EP standard compliance.
In short:
UV: Fast, economical, for total silymarin content.
HPLC: Precise, component-specific, for strict quality standards.
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